![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3981015:Design
Designed by: Yulin Peng Group: iGEM21_AHUT_China (2021-10-14)
pET28a-his-linker-her2-linker-ISZ-sTRAIL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The codon may be interchanged, and the splicing site may become invalid during the synthesis link process.
Source
On the basis of his-linker-ISZ-sTRAIL, the expression of Her2 artificial antibody protein is added. We designed the sequence and restriction site, and searched for the relevant sequence on NCBI. After codon optimization, we asked the company to help us synthesize this plasmid.