Composite

Part:BBa_K3981015:Design

Designed by: Yulin Peng   Group: iGEM21_AHUT_China   (2021-10-14)


pET28a-his-linker-her2-linker-ISZ-sTRAIL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon may be interchanged, and the splicing site may become invalid during the synthesis link process.


Source

On the basis of his-linker-ISZ-sTRAIL, the expression of Her2 artificial antibody protein is added. We designed the sequence and restriction site, and searched for the relevant sequence on NCBI. After codon optimization, we asked the company to help us synthesize this plasmid.

References